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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
02/03/2017 |
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Actualizado : |
07/03/2017 |
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Autor : |
GARCIA, J.P; GIANNITTI, F.; FINNIE, J.W.; MANAVIS, J. .; BEINGESSER, J.; ADAMS ,V.; ROOD, J.I.; UZAL, F.A. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Comparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Veterinary Pathology, 2015, v.52.n.6. p.1250-1253, 2015. |
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Idioma : |
Inglés |
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Contenido : |
Abstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease. MenosAbstract
Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated wi... Presentar Todo |
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Thesagro : |
ENFERMEDADES DE LOS ANIMALES. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02512naa a2200217 a 4500
001 1056750
005 2017-03-07
008 2015 bl uuuu u00u1 u #d
100 1 $aGARCIA, J.P
245 $aComparative Neuropathology of Ovine Enterotoxemia Produced by Clostridium perfringens Type D Wild-Type Strain CN1020 and Its Genetically Modified Derivatives.$h[electronic resource]
260 $c2015
520 $aAbstract Clostridium perfringens type D causes enterotoxemia in sheep and goats. The disease is mediated by epsilon toxin (ETX), which affects the cerebrovascular endothelium, increasing vascular permeability and leading to cerebral edema. In the present study, we compared the distribution and severity of the cerebrovascular changes induced in lambs by C. perfringens type D strain CN1020, its isogenic etx null mutant, and the ETX-producing complemented mutant. We also applied histochemical and immunohistochemical markers to further characterize the brain lesions induced by ETX. Both ETX-producing strains induced extensive cerebrovascular damage that did not differ significantly between each other in nature, neuroanatomic distribution, or severity. By contrast, lambs inoculated with the etx mutant or sterile, nontoxic culture medium did not develop detectable brain lesions, confirming that the neuropathologic effects observed in these infections are dependent on ETX production. Lambs treated with the wild-type and complemented strains showed perivascular and mural vascular edema, as well as serum albumin extravasation, particularly severe in the cerebral white matter, midbrain, medulla oblongata, and cerebellum. Brains of animals inoculated with the ETX-producing strains showed decreased expression of glial fibrillary acidic protein and increased expression of aquaporin-4 in the end-feet processes of the astrocytes around blood vessels. Early axonal injury was demonstrated with anti?amyloid precursor protein immunohistochemistry. Perivascular accumulation of macrophages/microglia with intracytoplasmic albumin globules was also observed in these animals. This study demonstrates that ETX is responsible for the major cerebrovascular changes in C. perfringens type D?induced disease.
650 $aENFERMEDADES DE LOS ANIMALES
700 1 $aGIANNITTI, F.
700 1 $aFINNIE, J.W.
700 1 $aMANAVIS, J. .
700 1 $aBEINGESSER, J.
700 1 $aADAMS ,V.
700 1 $aROOD, J.I.
700 1 $aUZAL, F.A.
773 $tVeterinary Pathology, 2015$gv.52.n.6. p.1250-1253, 2015.
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
19/06/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
GASO, D.; CAPURRO, M.C.; MORALES, M.; CALISTRO, R.; SILVA, L.; SCHUSSELIN, J.; SAWCHIK, J. |
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Afiliación : |
DEBORAH VIVIANA GASO MELGAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA CRISTINA CAPURRO BAZZANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA XIMENA MORALES LORENZONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ARIEL CALISTRO DIAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LEONARDO DANIEL SILVA INGOLD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MARCELO SCHUSSELIN DAMBURIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE SAWCHIK PINTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Manejo del arreglo espacial en soja de 1a. y 2a. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
In: Día de Campo Manejo de Cultivos de Verano en Secano y Bajo Riego (2014, INIA La Estanzuela, Uruguay). |
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Páginas : |
p. 9-18. |
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Serie : |
(Serie Actividades de Difusión; 729). |
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Idioma : |
Español |
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Palabras claves : |
DENSIDAD DE PLANTAS EN SOJA; ESPACIAMIENTO ENTRE HILERAS. |
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Thesagro : |
GLYCINE MAX; SOJA. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10396/1/SAD-729.p.9-18-Gaso-et-al.pdf
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Marc : |
LEADER 00745naa a2200253 a 4500
001 1036686
005 2018-06-19
008 2014 bl uuuu u00u1 u #d
100 1 $aGASO, D.
245 $aManejo del arreglo espacial en soja de 1a. y 2a.
260 $c2014
300 $ap. 9-18.
490 $a(Serie Actividades de Difusión; 729).
650 $aGLYCINE MAX
650 $aSOJA
653 $aDENSIDAD DE PLANTAS EN SOJA
653 $aESPACIAMIENTO ENTRE HILERAS
700 1 $aCAPURRO, M.C.
700 1 $aMORALES, M.
700 1 $aCALISTRO, R.
700 1 $aSILVA, L.
700 1 $aSCHUSSELIN, J.
700 1 $aSAWCHIK, J.
773 $tIn: Día de Campo Manejo de Cultivos de Verano en Secano y Bajo Riego (2014, INIA La Estanzuela, Uruguay).
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